elisa试剂盒的文献翻译

酶联免疫吸附试验（ELISA）：ELISA是“酶联免疫吸附测定法。”这是一个快速的免疫化学试验，涉及一种酶（一种蛋白质，催化生化反应）。它还涉及抗体或抗原（免疫分子）。
酶联免疫吸附试验是用来检测具有抗原特性的物质，主要是蛋白质（相对于小分子和离子，如葡萄糖和钾）。其中包括激素，细菌抗原和抗体。

elisa试剂盒的文献翻译

这个试验有变异，但zui基本的是附在固体表面的抗体。这种抗体的亲和力（将锁上）例如，利益，物质，人绒毛膜促性腺激素（HGC），常用的测量表明妊娠蛋白质。将纯化的绒毛膜促性腺激素（偶联）的混合物与酶和测试样品（血液、尿液等）加入到测试系统中。如果没有HCG在测试样品中存在，那么只有绒毛膜促性腺激素与链接酶将结合。在测试样本中存在的HCG越多，与绒毛膜结合的绒毛膜促性腺激素结合的可能性就越小。然后添加酶作用的物质，并以某种方式测量产品的量，如溶液颜色的变化。
ELISA检测通常是高度敏感和特异的与放射免疫法（RIA）试验比较。他们有优势，不需要放射性同位素和辐射计数装置。

elisa试剂盒的文献翻译

Enzyme-linked immunosorbent assay (ELISA): ELISA stands for "enzyme-linked immunosorbent assay." This is a rapid immunochemical test that involves an enzyme (a protein that catalyzes a biochemical reaction). It also involves an antibody or antigen (immunologic molecules).

ELISA tests are utilized to detect substances that have antigenic properties, primarily proteins (as opposed to small molecules and ions such as glucose and potassium). Some of these include hormones, bacterial antigens and antibodies.

There are variations of this test, but the most basic consists of an antibody attached to a solid surface. This antibody has affinity for (will latch on to) the substance of interest, for example, human chorionic gonadotropin (HGC), the commonly measured protein which indicates pregnancy. A mixture of purified HCG linked (coupled) to an enzyme and the test sample (blood, urine, etc) are added to the test system. If no HCG is present in the test sample, then only HCG with linked enzyme will bind. The more HCG which is present in the test sample, the less enzyme linked HCG will bind. The substance the enzyme acts on is then added, and the amount of product measured in some way, such as a change in color of the solution.

ELISA tests are generally highly sensitive and specific and compare favorably with radioimmune assay (RIA) tests. They have the added advantages of not needing radioisotopes or a radiation-counting apparatus.

elisa试剂盒的文献翻译