tnf-a试剂盒的前世来生

tnf-a试剂盒影响因素

试验与测定方法

细胞因子主要是根据其生物学活性和免疫学检测，生物试验是确定生物活性和免疫学检测是测定蛋白质的重量或体积浓度。这两种信息对于了解细胞因子在疾病中的作用是非常重要的。由于总蛋白量的细胞因子和免疫根据检测的生物活性的测定可能会有所不同，所以在观察时应注意不同的方法不能比较。

2。细胞因子免疫学检测方法主要采用酶联免疫吸附法或放射免疫法。不同试剂盒对样品有不同的要求，标本按照试剂盒说明书的要求保存

[influencing factors]
Test and determination method of 1. cytokines is mainly based on their biological activity and immunological tests, biological tests is to determine the biological activity and immunological test is the determination of the weight or volume of protein concentration. These two kinds of information are important for understanding the role of cytokines in disease. Due to the total protein amount of cytokines and immune according to the inspection by the determination of biological activity may be different, so in the clinical observation should pay attention to different methods can not be compared.

[意义] TNF主要由巨噬细胞和单核细胞产生。人们已经发现，TNF-α可以产生多器官的生物学效应，更重要的是在substance.tnf功能在细胞因子网络具有多种生物学效应，主要是通过抗肿瘤和免疫调节功能的免疫介导的，也是炎症反应的介质，在炎症性病变的病理变化许多方面。血清或其他体液中的TNF浓度，是基础医学和疾病诊断和治疗价值的指标。

1。肿瘤：许多肿瘤细胞可以产生肿瘤坏死因子，因此在许多肿瘤时，肿瘤坏死因子表达明显增加，肿瘤坏死因子可以杀死肿瘤细胞，或通过cytotoxicity

2抑制某些肿瘤细胞的增殖。类风湿关节炎患者有大量TNF。在他们的synovium3。在脓毒症、肺炎等严重炎症性疾病时血清TNF含量显著升高。肿瘤坏死性寄生虫病患者明显改变。TNF患者体液免疫患者高于正常人。疟疾抗原，病毒和细菌可以诱导肿瘤坏死因子TNF生物试剂，反过来，抗病毒，抗细菌，抗疟疾role.4.tnf是移植排斥反应密切相关，与肿瘤坏死因子的表达与血清或尿液排斥程度呈正相关
 

2. the immunological test method of cytokines mainly uses ELISA or RIA. Different reagent kits have different requirements for the samples, and the specimens are kept in accordance with the requirements of the kit instructions
[clinical significance]
TNF is mainly produced by macrophages and monocytes. It has been found that TNF can produce biological effects on many organs, it is more important in the function of substance.TNF in the cytokine network has a variety of biological effects, is mainly mediated by the immune function of anti-tumor and immune regulation, also is one of the mediators of inflammatory responses, many aspects of pathology the changes in inflammatory lesions. Serum or other body fluids in the concentration of TNF, is the basic medicine and clinical diseases diagnosis and treatment value of the index.
1. tumor: many tumor cells can produce TNF, so in many tumor time, TNF expression is obviously increased,.TNF can kill tumor cells or inhibit the proliferation of some tumor cells through cytotoxicity
2. rheumatoid arthritis patients have large amounts of TNF. in their synovium
3. in sepsis, pneumonia and other serious inflammatory diseases when the content of TNF in serum was significantly increased. Many patients with parasitic diseases in TNF was significantly changed. The humoral AIDS patients in TNF was higher than that of normal people. Malarial antigen, virus and bacteria can induce the production of TNF, TNF in turn, anti-virus, anti bacteria, anti malaria role.
4.TNF is closely related to graft rejection, and the expression of TNF is positively correlated with the extent of rejection in serum or urine