蓝色葡聚糖2000/Dextran Blue 2000*

蓝色葡聚糖2000/Dextran Blue 2000*

CAS Number: 87915-38-6

Storage Temperature: Room Temperature

Product Description :

The most common use for Blue Dextran is in the determination of the void volume in gel filtration chromatography columns. It is typically used at 1 mg/ml to 2 mg/ml in a buffer such as 50 mM Tris-HCl, pH 7.5 containing 100 mM KCl. The volume of the Blue Dextran sample should be 1% to no more than 3% of the column's bed volume. The shape of the Blue Dextran band as it elutes through the column can also be used to assess the column packing and resolution.

Blue Dextran has also been used as a spectrophotometric substrate for dextranase. Dextranase activity is measured by the increase in absorbance at 610 nm.

The affinity of the Reactive Blue 2 Dye in Blue dextran for certain proteins has been exploited in protein purification methods. When bound to a protein the Blue Dextran-protein complex will be eluted in the void volume of a sephadex gel filtration column. This allows the bound protein to be easily separated from other proteins of similar molecular weight. The binding of blue dextrans to proteins is dependent on pH and ionic strength.

Storage/Stability :

This product is given a shelf-life of five years from the date of manufacture. The solubility of Blue Dextran has been tested at 50 mg/ml in water. Dextran can be hydrolyzed by strong acids at high temperatures. The terminal reducing end group of dextran can be oxidized in alkaline solutions.Solutions should be prepared fresh when ever possible. Dextran is susceptible to microbial degradation. Solutions should be stored refrigerated.

Precautions and Disclaimer :

For Laboratory Use Only. Not for drug, household or other uses.